@article{Vaitinadapoule2024-fz,

title = {Nanotopography by chromatic confocal microscopy of the 

 endothelium in Fuchs endothelial corneal dystrophy, pseudophakic 

 bullous keratopathy and healthy corneas},

author = {Hanielle Vaitinadapoulé and Sylvain Poinard and Zhiguo He and Alina Pascale-Hamri and Justin Thomas and Philippe Gain and Jean-Yves Thuret and Frédéric Mascarelli and Gilles Thuret and French Fuchs Study Group (FFSG)},

year  = {2024},

date = {2024-07-01},

journal = {Br. J. Ophthalmol.},

volume = {108},

number = {8},

pages = {1184–1192},

publisher = {BMJ},

abstract = {AIM: To investigate the interest of chromatic confocal 

 microscopy (CCM) to characterise guttae in Fuchs endothelial 

 corneal dystrophy (FECD). METHODS: Descemet's membranes (DM) 

 were obtained during endothelial keratoplasty in patients with 

 FECD and pseudophakic bullous keratopathy (PBK). They were 

 compared with healthy samples obtained from body donation to 

 science. Samples were fixed in 0.5% paraformaldehyde and flat 

 mounted. Surface roughness of DMs was quantified using CCM and 

 the AltiMap software that provided the maximum peak (Sp) and 

 valley (Sv) heights, the mean square roughness (Rq) and the 

 asymmetry coefficient (Ssk). RESULTS: The physiological 

 roughness of healthy samples was characterised by an Rq of 

 0.12$±$0.05 µm, which was two times rougher than in PBK (Rq=0.06$±$0.03 µm), but both were still flat with a 

 symmetrical distribution between peaks and valleys (Ssk close to},

keywords = {Cornea; Dystrophy; Imaging; Pathology; Physiology},

pubstate = {published},

tppubtype = {article}

}